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arrayworx v.1.50 software  (Applied Precision Inc)

 
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    Applied Precision Inc arrayworx v.1.50 software
    Non-covalent adsorption of DNA oligonucleotides on a positively charged surface. (A) Standard curves for a 12mer (filled squares) and a 24mer (open squares). Cy5-labeled probes were printed at 10 nl, then imaged without washing on an <t>ArrayWorx</t> imager (Applied Precision). The number of probe molecules per array element (x-axis) was calculated from the product of printed volume, probe concentration and Avogadro’s number. The y-axis represents log of the mean integrated value of Cy5 dye fluorescent signal from the array elements. Each data point represents the mean and a single standard deviation from the mean calculated from 56 array elements. (B) Surface area per oligonucleotide occupied by a 12mer (filled circles) or a 24mer (open circles). Cy5-labeled oligonucleotide probe was printed in 70% DMSO/30% H2O at 10 nl/array element, as a function of concentration on the aminosilanized glass surface, followed by washing to remove unbound probe. The y-axis was calculated by dividing the measured array element surface area by the number of adsorbed probe molecules per array element [calculated from standard curves in (A)]. Each data point represents the mean and a single standard deviation from the mean calculated from 56 array elements.
    Arrayworx V.1.50 Software, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arrayworx v.1.50 software/product/Applied Precision Inc
    Average 90 stars, based on 1 article reviews
    arrayworx v.1.50 software - by Bioz Stars, 2026-05
    90/100 stars

    Images

    1) Product Images from "Oligonucleotides form a duplex with non-helical properties on a positively charged surface"

    Article Title: Oligonucleotides form a duplex with non-helical properties on a positively charged surface

    Journal:

    doi:

    Non-covalent adsorption of DNA oligonucleotides on a positively charged surface. (A) Standard curves for a 12mer (filled squares) and a 24mer (open squares). Cy5-labeled probes were printed at 10 nl, then imaged without washing on an ArrayWorx imager (Applied Precision). The number of probe molecules per array element (x-axis) was calculated from the product of printed volume, probe concentration and Avogadro’s number. The y-axis represents log of the mean integrated value of Cy5 dye fluorescent signal from the array elements. Each data point represents the mean and a single standard deviation from the mean calculated from 56 array elements. (B) Surface area per oligonucleotide occupied by a 12mer (filled circles) or a 24mer (open circles). Cy5-labeled oligonucleotide probe was printed in 70% DMSO/30% H2O at 10 nl/array element, as a function of concentration on the aminosilanized glass surface, followed by washing to remove unbound probe. The y-axis was calculated by dividing the measured array element surface area by the number of adsorbed probe molecules per array element [calculated from standard curves in (A)]. Each data point represents the mean and a single standard deviation from the mean calculated from 56 array elements.
    Figure Legend Snippet: Non-covalent adsorption of DNA oligonucleotides on a positively charged surface. (A) Standard curves for a 12mer (filled squares) and a 24mer (open squares). Cy5-labeled probes were printed at 10 nl, then imaged without washing on an ArrayWorx imager (Applied Precision). The number of probe molecules per array element (x-axis) was calculated from the product of printed volume, probe concentration and Avogadro’s number. The y-axis represents log of the mean integrated value of Cy5 dye fluorescent signal from the array elements. Each data point represents the mean and a single standard deviation from the mean calculated from 56 array elements. (B) Surface area per oligonucleotide occupied by a 12mer (filled circles) or a 24mer (open circles). Cy5-labeled oligonucleotide probe was printed in 70% DMSO/30% H2O at 10 nl/array element, as a function of concentration on the aminosilanized glass surface, followed by washing to remove unbound probe. The y-axis was calculated by dividing the measured array element surface area by the number of adsorbed probe molecules per array element [calculated from standard curves in (A)]. Each data point represents the mean and a single standard deviation from the mean calculated from 56 array elements.

    Techniques Used: Adsorption, Labeling, Concentration Assay, Standard Deviation

    Analysis of the washing eluate. (A) Patches of aminosilanized surface (3 mm2 each) were saturated with Cy5-labeled probes (red), followed by rinsing to remove excess probe as described in Materials and Methods. Cy3-labeled targets (green) were hybridized to these patches, rinsed to remove the unbound targets and washed in 2 µl of the washing buffer for 15 min (see Materials and Methods). A 0.2 µl aliquot was aspirated from the resulting washing buffer and spotted on a clean slide, which was subsequently imaged with Cy3 and Cy5 filter sets on an ArrayWorx Imager (Applied Precision). (B) The reverse experiments are also shown, where the targets and probes had been reversed. The bar graphs represent the normalized means and the standard deviations of the mean from four such 0.2 µl eluates.
    Figure Legend Snippet: Analysis of the washing eluate. (A) Patches of aminosilanized surface (3 mm2 each) were saturated with Cy5-labeled probes (red), followed by rinsing to remove excess probe as described in Materials and Methods. Cy3-labeled targets (green) were hybridized to these patches, rinsed to remove the unbound targets and washed in 2 µl of the washing buffer for 15 min (see Materials and Methods). A 0.2 µl aliquot was aspirated from the resulting washing buffer and spotted on a clean slide, which was subsequently imaged with Cy3 and Cy5 filter sets on an ArrayWorx Imager (Applied Precision). (B) The reverse experiments are also shown, where the targets and probes had been reversed. The bar graphs represent the normalized means and the standard deviations of the mean from four such 0.2 µl eluates.

    Techniques Used: Labeling



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    arrayworx v.1.50 software  (Applied Precision Inc)
    90
    Applied Precision Inc arrayworx v.1.50 software
    Non-covalent adsorption of DNA oligonucleotides on a positively charged surface. (A) Standard curves for a 12mer (filled squares) and a 24mer (open squares). Cy5-labeled probes were printed at 10 nl, then imaged without washing on an <t>ArrayWorx</t> imager (Applied Precision). The number of probe molecules per array element (x-axis) was calculated from the product of printed volume, probe concentration and Avogadro’s number. The y-axis represents log of the mean integrated value of Cy5 dye fluorescent signal from the array elements. Each data point represents the mean and a single standard deviation from the mean calculated from 56 array elements. (B) Surface area per oligonucleotide occupied by a 12mer (filled circles) or a 24mer (open circles). Cy5-labeled oligonucleotide probe was printed in 70% DMSO/30% H2O at 10 nl/array element, as a function of concentration on the aminosilanized glass surface, followed by washing to remove unbound probe. The y-axis was calculated by dividing the measured array element surface area by the number of adsorbed probe molecules per array element [calculated from standard curves in (A)]. Each data point represents the mean and a single standard deviation from the mean calculated from 56 array elements.
    Arrayworx V.1.50 Software, supplied by Applied Precision Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/arrayworx v.1.50 software/product/Applied Precision Inc
    Average 90 stars, based on 1 article reviews
    arrayworx v.1.50 software - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

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    Non-covalent adsorption of DNA oligonucleotides on a positively charged surface. (A) Standard curves for a 12mer (filled squares) and a 24mer (open squares). Cy5-labeled probes were printed at 10 nl, then imaged without washing on an ArrayWorx imager (Applied Precision). The number of probe molecules per array element (x-axis) was calculated from the product of printed volume, probe concentration and Avogadro’s number. The y-axis represents log of the mean integrated value of Cy5 dye fluorescent signal from the array elements. Each data point represents the mean and a single standard deviation from the mean calculated from 56 array elements. (B) Surface area per oligonucleotide occupied by a 12mer (filled circles) or a 24mer (open circles). Cy5-labeled oligonucleotide probe was printed in 70% DMSO/30% H2O at 10 nl/array element, as a function of concentration on the aminosilanized glass surface, followed by washing to remove unbound probe. The y-axis was calculated by dividing the measured array element surface area by the number of adsorbed probe molecules per array element [calculated from standard curves in (A)]. Each data point represents the mean and a single standard deviation from the mean calculated from 56 array elements.

    Journal:

    Article Title: Oligonucleotides form a duplex with non-helical properties on a positively charged surface

    doi:

    Figure Lengend Snippet: Non-covalent adsorption of DNA oligonucleotides on a positively charged surface. (A) Standard curves for a 12mer (filled squares) and a 24mer (open squares). Cy5-labeled probes were printed at 10 nl, then imaged without washing on an ArrayWorx imager (Applied Precision). The number of probe molecules per array element (x-axis) was calculated from the product of printed volume, probe concentration and Avogadro’s number. The y-axis represents log of the mean integrated value of Cy5 dye fluorescent signal from the array elements. Each data point represents the mean and a single standard deviation from the mean calculated from 56 array elements. (B) Surface area per oligonucleotide occupied by a 12mer (filled circles) or a 24mer (open circles). Cy5-labeled oligonucleotide probe was printed in 70% DMSO/30% H2O at 10 nl/array element, as a function of concentration on the aminosilanized glass surface, followed by washing to remove unbound probe. The y-axis was calculated by dividing the measured array element surface area by the number of adsorbed probe molecules per array element [calculated from standard curves in (A)]. Each data point represents the mean and a single standard deviation from the mean calculated from 56 array elements.

    Article Snippet: The analysis of intensities from the Cy3 and Cy5 channels was done with ArrayWorx v.1.50 software (Applied Precision) from the original stitched images and bar graphs were generated in Microsoft Excel.

    Techniques: Adsorption, Labeling, Concentration Assay, Standard Deviation

    Analysis of the washing eluate. (A) Patches of aminosilanized surface (3 mm2 each) were saturated with Cy5-labeled probes (red), followed by rinsing to remove excess probe as described in Materials and Methods. Cy3-labeled targets (green) were hybridized to these patches, rinsed to remove the unbound targets and washed in 2 µl of the washing buffer for 15 min (see Materials and Methods). A 0.2 µl aliquot was aspirated from the resulting washing buffer and spotted on a clean slide, which was subsequently imaged with Cy3 and Cy5 filter sets on an ArrayWorx Imager (Applied Precision). (B) The reverse experiments are also shown, where the targets and probes had been reversed. The bar graphs represent the normalized means and the standard deviations of the mean from four such 0.2 µl eluates.

    Journal:

    Article Title: Oligonucleotides form a duplex with non-helical properties on a positively charged surface

    doi:

    Figure Lengend Snippet: Analysis of the washing eluate. (A) Patches of aminosilanized surface (3 mm2 each) were saturated with Cy5-labeled probes (red), followed by rinsing to remove excess probe as described in Materials and Methods. Cy3-labeled targets (green) were hybridized to these patches, rinsed to remove the unbound targets and washed in 2 µl of the washing buffer for 15 min (see Materials and Methods). A 0.2 µl aliquot was aspirated from the resulting washing buffer and spotted on a clean slide, which was subsequently imaged with Cy3 and Cy5 filter sets on an ArrayWorx Imager (Applied Precision). (B) The reverse experiments are also shown, where the targets and probes had been reversed. The bar graphs represent the normalized means and the standard deviations of the mean from four such 0.2 µl eluates.

    Article Snippet: The analysis of intensities from the Cy3 and Cy5 channels was done with ArrayWorx v.1.50 software (Applied Precision) from the original stitched images and bar graphs were generated in Microsoft Excel.

    Techniques: Labeling